Contact an Account ManagerSeparation of 18 PAHs on NUCLEODUR
Summary
Type: HPLC
Application #: 123840
Catalog#: 760784.40 - MN HPLC Column, Nucleodur C18 PAH, 110A, 3um, 4x125mm
Detection:
Conditions: Eluent A: methanol / water (70:30, v/v)
Eluent B: acetonitrile
Gradient: 0-20 % B in 1.5 min, 20-50 % B in 1.5 min, 50-100 % B in 1.0 min, 100 % B for 3.0 min, 100 - 0 % B in 0.5 min
Flow rate: 1.5 ml/min
Temperature: 35 °C
Injection volumes:
UV, 1 µl (10 ng per compound)
Fluorescence, 0.5 µl (5 ng per compound)
Authors: HPLC department
Source: Macherey-Nagel, Germany 2010