Contact an Account ManagerDetermination of Perfluorinated Surfactants by LC-MS
Summary
Type: HPLC
Application #: 123340
Catalog#: 760807.20 - MN HPLC Column, Nucleodur Sphinx RP, 110A, 3um, 2x125mm
Detection:
Conditions: Eluent A: 10 mM amonium acetate in water : methanol, 75:25, v:v
Eluent B: 10 mM amonium acetate in acetonitrile : methanol, 75:25, v:v
Gradient: 10 % -> 30 % B in 3 min, 30 % -> 55 % B in 8 min,
55 % -> 10 % B in 4 min
Flow rate: 0.30 mL/min
Temperature: 50 °C
Injection volume: 2.5 µL (each concentration=5 mg/L, after SPE enrichment)
Authors: HPLC department
Source: Macherey-Nagel (Germany 2009)