122740 Determination of vitamins on NUCLEODUR

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Determination of vitamins on NUCLEODUR

Summary

Type: HPLC
Application #: 122740
Catalog#: 760201.40 - MN HPLC Column, Nucleodur C18 Pyramid, 110A, 5um, 4x125mm
Detection:
Conditions: Eluent: A) 50 mM KH2PO4, pH = 2.5 B) methanol / acetonitrile, 70:30 v,v Gradient: 0 % B for 5 min, 0-35 % B in 1 min, 35 % B for 5 min; 0 % for 5.5 min Flow rate: 0.6 ml/min Temperature: 40 °C Injection volume: 10 µl
Authors: HPLC department
Source: Macherey-Nagel, Germany 2008