122290 Separation of Proteins

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Separation of Proteins

Summary

Type: HPLC
Application #: 122290
Catalog#: 720065.40 - MN HPLC Column, Nucleosil C18, 300A, 5um, 4x250mm
Detection:
Conditions: Eluent: A) water with 0.1% TFA; B) acetonitrile with 0.08% TFA Gradient: 5-90% B in 60 min; 90-5% B in 1 min, 5% B for 4 min Flow rate: 1.0 ml/min Temperature: 20 °C Injection volume: 50 µl
Authors: HPLC department
Source: Macherey-Nagel, Germany 2007