121030 Determination of Glyphosate and Aminomethyl Phosphonic Acid (AMPA) in Water by HPLC Pre Column Derivatisation and Fluorescence Detection

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Determination of Glyphosate and Aminomethyl Phosphonic Acid (AMPA) in Water by HPLC Pre Column Derivatisation and Fluorescence Detection

Summary

Type: HPLC
Application #: 121030
Detection: Fluorescence, Ex.: 258 nm (band width 12.5 nm), Em.: 305 nm (band width 25.0 nm)
Conditions: Eluent: A) acetonitrile / B) 0.002 M KH2PO4 (250 mg/l), pH=7 Gradient: 7- 57% A in 25 min, 57 - 95% A in 2 min, 95% A for 8 min, 95 - 7% for 2 min, 7% A in 5 min Temperature: 25° C Flow rate: 0.5 m
Authors: Reupert R Schlett C
Source: Landesumweltamt NRW Essen Germany