121020 Separation of DNA rRNA and tRNA from Soil after Waste Water Treatment

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Separation of DNA rRNA and tRNA from Soil after Waste Water Treatment

Summary

Type: HPLC
Application #: 121020
Catalog#: 736601 - MN HPLC Column (Valco type), Nucleogen DEAE, 4000A, 7um, 6x125mm
Detection: UV, 260 nm
Conditions: Eluent A: 5.0 M urea + 0.1 M KH2PO4 + 0.1 M K2HPO4
Eluent B: Eluent a + 1.5 M KCl
Eluent C: 0.01% NaN3

Gradient: see table below

Temperature: 23° C
Authors: Schwarz M
Source: Inst f Siedlungswasserwirtschaft Universität Karlsruhe Germany (2004)