Contact an Account ManagerSeparation of Derivatised Amino Acids
Summary
Type: HPLC
Application #: 120510
Catalog#: 760001.40 - MN HPLC Column, Nucleodur C18 ec, 100A, 5um, 4x125mm
Detection: Fluorescence, Ex 230 nm, Em 450 nm
Conditions: Eluent: A: methanol / acetonitrile (50:50 v/v);
B: 40 mM sodium acetate buffer, pH 6.5 + 5% A
gradient: 93% B for 5 min, 93 - 90% B in 2 min, 90 - 85% B in 8 min, 85 - 50% B in 7 min, 50 - 0% B in 3 min, 0% B for 3 min, 0 - 20% B in 1 min, 20 - 50% B in 1 min, 50 - 80% B in 1 min, 80 - 93% in 1 min.
Flow rate: 0.8 ml/min for 3.1 min, then 1 ml/min
Temperature: 35° C
Inj. volume: 5 μl
Authors: Wirtz
Source: Institut für Biochemie Köln Germany