Contact an Account ManagerQuality Control of Protein Samples by LC-MS Analysis
Summary
Type: HPLC
Application #: 120340
Detection: UV (214 nm) in row with MS (single quadrupole, 1:10 flow splitter, ESI, positive ion mode, full scan 600 - 1800 m/z, 2.7 s/scan)
Conditions: Eluent: A: 0.1% TFA in water, B: 0.1% TFA in acetonitrile
Gradient: 5 - 95% B in 15 min; For samples with low salt or with very hydrophobic proteins a gradient start with 20% B is recommendable.
Flow rate: 250 μl/min
Injection volume: 10 μl
Authors: Fischer B Siedler F
Source: Int Laboratory 8/2004 Vol 34 No 4 18-20
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