119470 Separation of trimethoprim and sulfamethoxazole, part III

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Separation of trimethoprim and sulfamethoxazole, part III

Summary

Type: HPLC
Application #: 119470
Catalog#: 760152.40 - MN HPLC Column, Nucleodur CN-RP, 100A, 5um, 4x250mm
Detection: UV 220 nm
Conditions: Injection: 10 μl
Eluents: Methanol / Buffer 40:60 (v/v)
Buffer: 10 mM Na-hexansulfonat + 100 mM KH2PO4 (pH 3.1)
Flow rate: 1.0 ml/min
Temperature: 30°C
Pressure: 150 bar
Authors: HPLC department
Source: Macherey-Nagel