118640 Analysis of p-Aminohippuric Acid and its Metabolite from Plasma

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Analysis of p-Aminohippuric Acid and its Metabolite from Plasma

Summary

Type: HPLC
Application #: 118640
Detection: UV DAD, 273 nm for 5 min, then 265 nm
Conditions: Eluent:
A: 7.88 g/l citric acid monohydrate, 3 g/l NaOH, 1 g/l Na heptanesulfonate monohydrate, adjusted to pH 3.9 with 2.7 ml 85% H3PO4
B: methanol
Gradient: 1 - 15% B in 15 min
Flow rate: 1 ml/min
Inj. volume: 25 μl

Authors: Decosterd L A et al
Source: J Chromatogr 703 (1997) 25-36