118010 Separation of Basic Alkaloids at pH 10.0

Contact an Account Manager

Separation of Basic Alkaloids at pH 10.0

Summary

Type: HPLC
Application #: 118010
Catalog#: 760100.40 - MN HPLC Column, Nucleodur C18 Gravity, 110A, 5um, 4x125mm
Detection: UV 254 nm
Conditions: Mobile phase: A: Acetonitrile, B: 20 mM (NH4)2HPO4, pH 10.0
Gradient:
0 min 10% A / 90% B --> 1 min --> 10%A / 90% B
--> 10 min --> 75% A / 25% B
Flowrate: 1.0 ml/min
Temperature: 25°C
Sample size: 2 μl
Authors: Macherey-Nagel
Source: HPLC Department


Details


Legend: 1) Papverine 2) Lidocaine 3) Noscapine 4) Diphenhydramine