117790 Separation of Antidepressants with NUCLEODUR

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Separation of Antidepressants with NUCLEODUR

Summary

Type: HPLC
Application #: 117790
Catalog#: 760100.40 - MN HPLC Column, Nucleodur C18 Gravity, 110A, 5um, 4x125mm
Detection: UV 230 nm
Conditions: Mobile phase: A) Methanol / Acetonitrile 50:50 (v/v)
B) 20 mM KH2PO4, pH 7.0
Gradient:
A / B 50 / 50 --> 6 min --> A / B 65 / 35
A / B 65 / 35 --> 20 min --> A / B 65 / 35

Flowrate: 1.2 ml/min
Temperature: 30°C
Sample size: 5 μl
Authors: Macherey-Nagel
Source: HPLC department