117730 Liquid chromatographic analysis of supercritical carbon dioxide extracts of Schizandra chinensis

Contact an Account Manager

Liquid chromatographic analysis of supercritical carbon dioxide extracts of Schizandra chinensis

Summary

Type: HPLC
Application #: 117730
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV 254 nm
Conditions: Eluents: A: CH3CN-H2O, B: MeOH-H2O

Gradient: Multistep gradients were used: A: 5 min 50:50 (v/v) isocratic; gradient 30 min 60:40 (v/v); gradient 20 min 70:30 (v/v); 15 min 70:30 (v/v) isocratic
B:1 min 70:30 (v/v) isocratic; gradient 34 min 95:5 (v/v); 5 min 95:5 (v/v) isocratic

Flow rate: 0,75 ml/min
Temperature: 18-20°C
Authors: Bártlová M Opletal L Chobot V Sovová H
Source: Journal of Chromatography B 770 (2002) 283-28