111270 Analysis of mono nucleotides in presence of inosine and hypoxanthine

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Analysis of mono nucleotides in presence of inosine and hypoxanthine

Summary

Type: HPLC
Application #: 111270
Detection:
Conditions: Eluents: A) 0.1 M KH2PO4, 4 mM tetrabutylammonium dihydrogen phosphate, pH 6.0
B) 50% A plus 50% methanol
Gradient: 0-6 min. 15% B, 6-16 min. from 15% to 90% B, 16-17 min. 90% B, 17-17.5 min. to 15% B,
17.5-31.5 min. 15% B, 32 min.: start of next run
Flow rate: 0.5 mL/min
Authors: HPLC department
Source: Macherey-Nagel