Contact an Account ManagerDetermination of aspirin and salicylic acid in transdermal perfusates
Summary
Type: HPLC
Application #: 110430
Catalog#: 720013.46 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4.6x250mm
Detection: DAD, 2.5-5 min. 210 nm, 5-9 min. 246 nm, 9-16 min. 278 nm, 16-23 min. 215 nm
Conditions: Eluents:
A) Water / acetonitrile / o-phthaldialdehyd (OPA) 650:350:2 (v/v/v) (pH 2.5 after addition of acetonitrile)
B) Acetonitrile / OPA 1000:2 (v/v)
Gradient: 0-6.5 min. A/B 100:0, 6.5-10.5 min. to A/B 70:30, 10.5-19 min. A/B 70:30 isocratic, 19-22 min. to A/B 100:0, 22-28 min. A/B 100:0 isocratic
Flow rate: 1 mL/min
Authors: McMahon GP
Source: J of Chromatograpy B 707 (1998) 322-327