110410 Determination of ( )-catechin in human plasma

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Determination of ( )-catechin in human plasma

Summary

Type: HPLC
Application #: 110410
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 225 nm
Conditions: Eluents:
A) 50 mmol (NH4)H2PO4 adjusted to pH 2.6 with orthophosphoric acid
B) 20 % of A) with 80 % acetonitrile
C) 0.2 mmol orthophosphoric acid adjusted to pH 1.5 with ammonia

Gradient:
0-5 min. A/B/C 100:0:0, 5-15 min. to A/B/C 96/4/0, 15-25 min. to A/B/C 92:8:0, 25.01 min. A/B/C 0:8:92, 25.01-45 min. to A/B/C 0:20:80, 45-50 min. to A/B/C 0:30:70, 50-55 min. to A/B/C 0:40:60, 55-60 min. to A/B/C 0:80:20, 60-65 min. to A/B/C 100:0:0

Flow rate: 0.7 mL/min
Authors: Carando S
Source: J of Chromatography B 707 (1998) 195-201


Details


Legend: Benzylpenicillin