Contact an Account ManagerAnalysis of phenylthiohydantoin (PTH) amino acids
Summary
Type: HPLC
Application #: 110370
Catalog#: 720002.20 - MN HPLC Column, Nucleosil C18, 100A, 5um, 2x125mm
Detection: RI, attenuation 27
Conditions: Eluents:
A) 40 ml PE-Applied Premix Buffer, 1000 ml (H2O + 3.5 % THF),
B) 10 % i-propanol in in acetonitrile
Gradient:
A/B 92:8, 0 min. 0.4 min. A/B 89:11, 10 min. A/B 65:35, 17 min. isocratic A/B 65:35, 18 min. A/B 10:90, 20 min. isocratic A/B 10:90
Flow rate: 0.325 mL/min
Temperature: 55 °C
Authors: Raida M
Source: Institut für Peptidforschung Hannover Germany