110240 Analysis of morphine, benzoylecgonine, cocaine, codeine from serum

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Analysis of morphine, benzoylecgonine, cocaine, codeine from serum

Summary

Type: HPLC
Application #: 110240
Detection: UV, 210 nm
Conditions: Eluents:
A) 2 mmol NaH2PO4, 1 g 1-hexanesulfonic acid adjusted with H3PO4 to pH 2,5
B) Acetonitril

Gradient: A/B 88:12, in 30 min. to 67:33, then for 5 min. 10:90

Inj. vol.: 60 ul
Flow rate: 0.35 mL/min.
Temperature: 40 °C
Authors: Kothe
Source: Laboratory Dr. Krone, Prof. Hagedorn, Herford