110220 Analysis of glutathione red/08 after derivat. with Sanger`s reagent

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Analysis of glutathione red/08 after derivat. with Sanger`s reagent

Summary

Type: HPLC
Application #: 110220
Detection: UV, 380 nm
Conditions: Eluents:
A) 2 mmol NaH2PO4, 1 g 1-hexanesulfonic acid adjusted with H3PO4 to pH 2,5
B) Acetonitril

Gradient: A/B 10:90, in 15 min. to 65:35, in 5 min. to 45:55, then for 5 min. 10:90
Inj. vol.: 20 ul
Flow rate: 0.25 mL/min.
Temperature: 40 °C
Authors: Kothe
Source: Laboratory Dr Krone Prof Hagedorn Herford Germany