Contact an Account ManagerDetermination of ascorbic acid and uric acid in plasma by HPLC
Summary
Type: HPLC
Application #: 109650
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV detection at 262 nm.
Conditions: mobile phase 25 mM myristyltrimethylammonium bromide as ion-pairing reagent
0.05 M sodium hydroxide, 0.06 M acetic acid, 7.5 % (v/v) acetonitrile, pH 5.5.
Homocysteine at 100 mg/l and EDTA at 200 mg/l were added before use
The system was operated at ambient temperature with a flow rate of 0.55 ml/min
Authors: Ross MA
Source: J Chromatogr B 657 (1994) 197 - 200
©2020 Copyright, All Rights Reserved, Sorbent Technologies, Inc.