109580 HPLC analysis of thiamine in rice flour with fluorimetric postcolumn derivatization

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HPLC analysis of thiamine in rice flour with fluorimetric postcolumn derivatization

Summary

Type: HPLC
Application #: 109580
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence, excitation 375nm, emission 435nm
Conditions: eluent: 0.01M NaH2PO4 + 0.15M NaClO4 adjusted to pH 2.2 with HClO4
flow rate 0.6ml/min
temperature 55°C
Authors: Ohta H
Source: J Chromatogr 284 (1984) 281 - 284


Details


Legend: Fig. 2. Chromatograms of A) thiamine standard (0.5µg/ml), and thiamine extracts of B) 90% polished rice flour, C) 93% polished rice flour, and D) brown rice flour of Sasanishiki cultivar, 25µl injec