109510 Separation of structural isomers of vitamin A acetate via coupling of HPLC and H-NMR

Contact an Account Manager

Separation of structural isomers of vitamin A acetate via coupling of HPLC and H-NMR

Summary

Type: HPLC
Application #: 109510
Catalog#: 720090.46 - MN HPLC Column, Nucleosil CN, 100A, 5um, 4.6x250mm
Detection: UV 325 nm
Conditions: Eluent: n-heptane (dried over molecular sieve 0.3nm)
flow rate: 20 min 1.0 ml/min (130 bar), then 0.2 ml/min (30 bar)
Authors: Albert K
Source: Angew Chem 107 (1995) 1102 - 1104


Details


Legend: Fig. 3: UV chromatogram of an isomer separation of vitamin A acetate (5 mg isomer mixtrue dissolved in 50 µl n-heptane). Peaks: 1. 11,13-di-cis-, 2. 11-cis, 3. 13-cis-, 4. 9-cis-, 5. all-trans-vitam