109350 HPLC of hydroxysteroids detected with postcolumn immobilized enzyme reactors
Home sorbtech.com Back-
Contact an Account Manager
HPLC of hydroxysteroids detected with postcolumn immobilized enzyme reactors
Summary
Type: HPLCApplication #: 109350
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence, excitation 340nm, emission 470nm
Conditions: eluent: 70% methanol in water
flow rate 1ml/min
reagent buffer: 0.3mM NAD, 10mM KH2PO4, 1mM Na2EDTA and 2mM N-acetylcysteine; mixing ratio of effluent to reagent buffer = 1:2
Authors: Lam, S. et al.
Source: J. Chromatogr. 441 (1988) 81–87
Details
Legend: Fig. 1. Chromatogram of 3b-hydroxysteroids, five-component mixture containing 5ng/µl of each steroid, chromatograms from bottom upwards correspond to 5, 10 and 20µl samples.