109330 Selective effects of reversed phase column packings in HPLC of steroids

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Selective effects of reversed phase column packings in HPLC of steroids

Summary

Type: HPLC
Application #: 109330
Catalog#: 720141.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x100mm
Detection: UV, 240nm
Conditions: eluent: exponential gradient from 32 to 100% acetonitrile in water with y = x3 over 50min
temperature 45°C
Authors: Nice EC
Source: J Chromatogr 166 (1978) 263 - 267


Details


Legend: Fig. 1. HPLC separation of steroid hormones, 1. 17ahydroxy-20a-dihydroprogesterone, 2. testosterone, 3. androstenedione, 4. 17a-hydroxyprogesterone, 5. 20a-dihydroprogesterone, 6. progesterone.