109270 Determination of natural cholesterone in rat adrenal extracts by HPLC

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Determination of natural cholesterone in rat adrenal extracts by HPLC

Summary

Type: HPLC
Application #: 109270
Catalog#: 720141.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x100mm
Detection: detection: UV, 244nm
Conditions: eluent: methanol - dichloromethane - water (96:2:2)
flow rate 0.9ml/min
Authors: Tallova J
Source: J Chromatogr 202 (1980) 487 - 490


Details


Legend: Fig. 1. Chromatograms of an extract of 1) the normal rat adrenal, 2) the adrenal of a rat fed for 7days with 0.25g cholestenone per day. Fig. 2. Chromatograms of 1) 100ng cortisol and 20ng corticost