108600 Use of HPLC to separate very small quantities of radiolabelled metabolites from biological tissue
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Use of HPLC to separate very small quantities of radiolabelled metabolites from biological tissue
Summary
Type: HPLCApplication #: 108600
Catalog#: 720090.40 - MN HPLC Column, Nucleosil CN, 100A, 5um, 4x250mm
Detection: UV, 280nm, 0.5 AUFS UV, 300nm, 0.5 AUFS
Conditions: eluent: 10% ether in hexane + 0.03% triethylamine for 2min, the gradient to ether + 0.3% triethylamine, 10% per min, flow rate 100ml/h; pressure 3000psi
(b) elution of compound III from the precolumn: eluent: ether + 0.5% acetic acid, flow rate 1ml/min; pressure 250psi
Authors: Rickett FE
Source: J Chromatogr 142 (1977) 705 - 713
Details
Legend: Fig. 3. (a) Chromatogram of amitraz and some potential metabolites, injection volume 10µl diisopropyl ether containing 200µg/ml amitraz, 400µg/ml I, 1mg/ml II, and 50µg/ml III.