108570 Isolation of high specific activity subunits of cholera toxin by reversed phase HPLC

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Isolation of high specific activity subunits of cholera toxin by reversed phase HPLC

Summary

Type: HPLC
Application #: 108570
Catalog#: 720022.46 - MN HPLC Column, Nucleosil C8, 100A, 10um, 4.6x250mm
Detection: UV, 230nm
Conditions: eluent A: 0.1% trifluoroacetic acid (TFA) in water, eluent B: 0.1% TFA in acetonitrile, 12min eluent A, then gradient from 0 to 25% B in 6min, from 25 to 60% B in 62min, and from 60 to 0% B in 10min
Authors: Pearson SD
Source: J Chromatogr 359 (1986) 413 - 421


Details


Legend: Fig. 2. Fractionation of carboxymethylated cholera toxin chains, a) reduced and carboxymethylated cholera toxin (100µg), B) reduced and carboxymethylated A subunit (25µg), C) reagent blank from the