108500 Analysis and purification of the synthetic acyl carrier protein ACP(65-74)

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Analysis and purification of the synthetic acyl carrier protein ACP(65-74)

Summary

Type: HPLC
Application #: 108500
Catalog#: 719456 - VA 150/4.6 NUCLEOGEL RP 100-8
Detection: UV, 220nm
Conditions: 1: eluent A: 0.1% trifluoroacetic acid (TFA) in acetonitrile - water (1 : 99), eluent B: 0.1% TFA in acetonitrile - water (99 : 1), linear gradient from 10 - 60% B in 20min
flow rate 1ml/min
2: eluent: 0.1% TFA in acetonitrile - water (2:8)
flow rate 1ml/min
Authors: LC department
Source: Macherey-Nagel


Details


Legend: 1. Gradient separation of 1.) ACP(66-74)(H-Gln) 2.) ACP(65-74) 3.) ACP(66-74)(Glp) 4.) thioanisole. 2. Isocratic purification of synthetic ACP(65-74) sample: a) 0.0046 mg b) 0.092 mg 3. Iso