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Immobilized Fe3 affinity chromatographic isolation of phosphopeptides
Summary
Type: HPLCApplication #: 108480
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: UV, 220nm
Conditions: temperature 40°C
eluents: A 0.115% trifluoroacetic acid (TFA), B 0.1% TFA - 60% acetonitrile, gradient as indicated by dotted line
flow rate 1ml/min
Authors: Scanff, P. et al.
Source: J. Chromatogr. 539 (1991) 425–432
Details
Legend: Fig. 1. Superimposition of chromatograms of the tryptic digest (CTH), the bound fraction (BF) and the unbound fraction (UBF).