108420 Improved recovery of ovalbumin by reversed phase HPLC

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Improved recovery of ovalbumin by reversed phase HPLC

Summary

Type: HPLC
Application #: 108420
Detection: UV, 280nm
Conditions: eluent A: 0.1M NaH2PO4 adjusted to pH 2.0 with 85% H3PO4, eluent B: isopropanol - ethylene glycol - eluent A (3:1:1, v/v) pH 2.0, linear gradient from A to B with 5% per min
flow rate 1.0ml/min
pressure 20 - 100 kg/cm2
temperature 15°C
Authors: Hayakawa K
Source: J Chromatogr 256 (1983) 172 - 175


Details


Legend: Fig. 1. Separation of polypeptides including commercial ovalbumin 1.) (Met5)-enkephalin (11.2µg) 2.) (Leu5)-enkephalin (11.8µg) 3.) (Tyr1)-somatostatin (5.2µg) 4.) human serum albumin (65µg) 5.)