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HPLC separation of the 4 subunits of phosphorylase kinase
Summary
Type: HPLCApplication #: 108390
Catalog#: 720059.40 - MN HPLC Column, Nucleosil C4, 300A, 5um, 4x250mm
Detection: UV, 214nm
Conditions: eluent A: 0.1% trifluoroacetic acid (TFA) in water, eluent B: acetonitrile - water (84:16, v/v) + 0.08% TFA, injection with 20% B, 3 min 20% B, from 20 to 50% B in 2min, from 50 to 70% B in 12min, 6min at 70% B, in 0 min to 76% B, 6min at 76% B, in 1min to 100% B
flow rate 1ml/min
Authors: Meyer HE
Source: Inst Physiol Chem Univ Bochum Germany 1987
Details
Legend: HPLC of rabbit muscle phosphorylase kinase (4 subunits).