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Purification of monoclonal antibody of undialyzed ascites fluid
Summary
Type: HPLCApplication #: 108360
Detection: UV, 280nm
Conditions: eluent A: 10mM TRIS / HCl pH 8.2, eluent B: A) + 1.5M NaCl, gradient from 0 to 13% B in 5min, then from 13 to 70% B in 0.5min
flow rate 1ml/min
pressure 50bar
Authors: LC department
Source: Macherey-Nagel
Details
Legend: Separation of ascites (46.3mg/ml of total protein, sample volume 5µl).