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Separation of protein standards
Summary
Type: HPLCApplication #: 108310
Detection: UV, 280nm, 0.08 AUFS
Conditions: eluent A: 2mM TRIS / acetate pH 8.0, eluent B: 20mM TRIS / acetate pH 8.0 + 1.5M KCl, linear gradient from 0 to 40% B in 20min flow rate 1ml/min pressure 76bar
Authors: Macherey-Nagel
Source: LC department
Details
Legend: Separation of protein standards, 1. catalase, 2. myoglobin, 3. a-amylase, 4. transferrin, 5. a-lactalbumin, 6. glucose oxidase, 7. trypsininhibitor soybean, sample volume 20µl.