108250 Reversed phase separations of proteins

Contact an Account Manager

Reversed phase separations of proteins

Summary

Type: GPC
Application #: 108250
Catalog#: 719460 - VA 50/4.6 NUCLEOGEL RP 300-8
Detection: UV, 220nm
Conditions: a) eluent A: 0.1% trifluoroacetic acid (TFA) in water; eluent B: 0.1% TFA in acetonitrile - water (95 : 5), linear gradient from 20 - 60% B in 22min
flow rate 1.5ml/min

b) eluent A: 0.1% TFA in acetonitrile - water (5:95, v/v), eluent B: 0.1% TFA in acetonitrile - water (95:5, v/v), linear gradient 18 - 60% B in 60s
flow rate 4ml/min
Authors: LC department
Source: Macherey-Nagel


Details


Legend: A) Separation of 1. ribonuclease, 2. insulin, 3. cytochrome c, 4. lysozyme, 5. bovine serum albumin, 6. myoglobin, 7. ovalbumin. B) Rapid separation of 1. ribonuclease A, 2. cytochrome c, 3. lysoz