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Analysis of human hemoglobin chains
Summary
Type: HPLCApplication #: 108240
Catalog#: 720245.40 - MN HPLC Column, Nucleosil C4 MPN, 300A, 5um, 4x250mm
Detection: UV, 220nm
Conditions: eluent A: 20% acetonitrile, 80% water, 0.1% trifluoroacetic acid (TFA), eluent B: 60% acetonitrile, 40% water, 0.1% TFA, linear gradient from 40 to 60% B in 60min
flow rate 1 ml/min
Authors: LC department
Source: Macherey-Nagel
Details
Legend: Separation of a hemolyzate of human blood, 1. Hem, 2. b-globin, 3. a-globin, 4. AgT-globin, 5. Gg-globin, 6. AgI-globin, injection volume 10µl.