108220 Separation of standard proteins - stability after treatment with alkaline eluent

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Separation of standard proteins - stability after treatment with alkaline eluent

Summary

Type: HPLC
Application #: 108220
Detection: UV, 280nm
Conditions: eluent A: 0.1% TFA in H2O, eluent B: 0.08% TFA in CH3CN, gradient from 20 to 60% B in 10min
flow rate 1.0ml/min
Authors: LC department
Source: Macherey-Nagel


Details


Legend: Separation of standard proteins 1. ribonuclease 2. cytochrome c 3. lysozyme 4. b-lactoglobulin 5. ovalbumin Left: before treatment, right: after treatment with 300 column volumes of the alkali