108210 HPLC of a hydrophilic standard protein mixture

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HPLC of a hydrophilic standard protein mixture

Summary

Type: HPLC
Application #: 108210
Detection: UV, 230nm
Conditions: eluent A: 0.1% TFA in water, eluent B: 80% acetonitrile in water + 0.1% TFA, conditioning about 10min with eluent A, after injection linear gradient from 0 to 40% B in 12min, from 40 to 65% B in 58min, 5min at 65% B, then in 5min from 65 to 0% B
flow rate 0.7ml/min
pressure 50bar
temperature 40°C
Authors: Macherey-Nagel
Source: LC department


Details


Legend: HPLC of a hydrophilic standard protein mixture: 1. cytochrome c (MW 12500), 2. lysozyme (14000), 3. trypsinogen (24000), 4. trypsininhibitor soybean (21500), 5. chymotrypsinogen (25000), 6. carboanh