108180 Micropreparative separation of peptides derived from sodium dodecyl sulphate-solubilized proteins

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Micropreparative separation of peptides derived from sodium dodecyl sulphate-solubilized proteins

Summary

Type: HPLC
Application #: 108180
Detection: UV, 215nm
Conditions: eluent A: 0.1% trifluoroacetic acid (TFA) in water, eluent B: 0.07% TFA in acetonitrile, linear gradient from 0 to 60% B in 60min flow rate 100µl/min
Authors: Bosserhoff A
Source: J Chromatogr 473 (1989) 71 - 77


Details


Legend: Fig. 1. HPLC of tryptic peptides (200pmol) derived from bovine serum albumin, sample A: no SDS, samples B and C: 50µg SDS, C treated with heptane - isoamyl alcohol (4:1, v/v) before injection.