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Synthesis of prothymosin a (ProTa) a 109 amino acid protein
Summary
Type: HPLCApplication #: 108170
Detection: UV, 210nm
Conditions: eluents: for a) to c) 5min 0.1% trifluoroacetic acid (TFA) in water, then gradient to 0.1% TFA in acetonitrile in 60min, for d) to f) 5min 0.06% TFA in water, then gradient to 0.054% TFA in acetonitrile in 60min
flow rate 1ml/min
Authors: Barlos K
Source: Angew Chem 103 (1991) 572 - 575
Details
Legend: Fig. 3. Chromatograms of a) crude deprotected ProTa(11-109), b) crude desacetyl-ProTa, c) crude mixture of ProTa(11-109) + ProTa(1-109) after acetylation, d) HPLC purified ProTa, e) tryptic digest o