108140 Processing of chromogranin B in bovine adrenal medulla

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Processing of chromogranin B in bovine adrenal medulla

Summary

Type: HPLC
Application #: 108140
Catalog#: 720065.40 - MN HPLC Column, Nucleosil C18, 300A, 5um, 4x250mm
Detection: UV, 214 nm
Conditions: eluent A: 0.05% trifluoroacetic acid in water
eluent B: 0.05% trifluoroacetic acid in acetonitrile
flow rate: 0.7 ml/min
gradient: 0 - 40% (B) over 35 min, 40 - 60% (B) over 10 min and 60 - 100% over 10 min.
Authors: Strub J-M
Source: Eur J Biochem 229 (1995) 356 - 368


Details


Legend: Fig. 4: Separation of short soluble intragranular CGB-derived fragments from bovine chromaffin granules and determination of the N-terminal amino acid sequence. (A) Schematic representation of bovin