108100 Reversed phase HPLC of human growth hormone

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Reversed phase HPLC of human growth hormone

Summary

Type: HPLC
Application #: 108100
Detection: UV, 215nm
Conditions: eluent A: a, b and c) (NH4)2HPO4 - NaH2PO4 adjusted to pH 2.5 with HClO4, d and e) (NH4)2HPO4 - H3PO4 pH 6.5, eluent B: 90% aqueous acetonitrile, linear gradient from 52 to 56% B in 60min
flow rate a, b and c, 1.0ml/min, d and e, 0.5ml/min
Authors: Welinder BS
Source: J Chromatogr 398 (1987) 309 - 316


Details


Legend: Fig. 1. Chromatograms of a) 20µg pituitary-derived hGH (phGH), b) 40µg biosynthetic hGH, c) and d) 50µg met-hGH - 22k hGH mixture, e) 2µg phGH.