108080 Chromatography of tryptic peptides of recombinant human growth hormone

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Chromatography of tryptic peptides of recombinant human growth hormone

Summary

Type: HPLC
Application #: 108080
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 214 nm
Conditions: temperature 35 °C
eluent: 5min isocratic 0.12% aqueous trifluoroacetic acid (TFA), then linear gradient to 38% acetonitrile in 60min, finally gradient to 57% acetonitrile over 10min
flow rate 1ml/min
Authors: Frenz J
Source: J Chromatogr 512 (1990) 299 - 314 and J Chromatogr


Details


Legend: Fig. 2. Tryptic map of 200µg of a biosynthetic human growth hormone digest mixture. Displacement chromatography for the isolation of minor components from the digest mixture is performed on two