107990 Separation of some polypeptide hormones by HPLC

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Separation of some polypeptide hormones by HPLC

Summary

Type: HPLC
Application #: 107990
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 220nm
Conditions: eluent: 0.005M tartrate buffer pH 3.0 - acetonitrile (70.8:29.2) containing 0.005M Na 1-butanesulphonate and 0.05 M Na2SO4
flow rate 1.0ml/min
Authors: Terabe S
Source: J Chromatogr 172 (1979) 163 - 177


Details


Legend: Fig. 16. Chromatograms of insulins, A) bovine insulin (Calbiochem, 3µg), B) purified bovine insulin (3µg), C) a mixture of purified [ThrB30]-bovine, bovine, human and porcine insulins.