107980 Improved HPLC method for the analysis of insulins and related compounds

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Improved HPLC method for the analysis of insulins and related compounds

Summary

Type: HPLC
Application #: 107980
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: UV, 215nm
Conditions: eluent A: methanol - acetonitrile - buffer pH 2.2 (5:1:4), eluent B: as A, but containing 0.01M Na2SO4, C as A but containing 0.1M Na2SO4
flow rate 1ml/min
Authors: Szepesi G
Source: J Chromatogr 218 (1981) 597 - 602 603 - 612


Details


Legend: Fig. 3. Separation of bovine and porcine insulins and proinsulins, 1. bovine insulin, 2. bovine deamidoinsulin, 3. porcine insulin, 4. bovine proinsulin, 5. porcine deamidoinsulin, 6. porcine proins