107970 Unexpected elution behaviour of peptides with reversed phase columns

Contact an Account Manager

Unexpected elution behaviour of peptides with reversed phase columns

Summary

Type: HPLC
Application #: 107970
Catalog#: 720051.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x125mm
Detection: UV, 220nm
Conditions: eluent: gradient from 28% aqueous acetonitrile, 0.1% trifluoroacetic acid (TFA) to 40% acetonitrile, 0.1% TFA in 30min
Authors: Engelhardt H
Source: J Chromatogr 499 (1990) 165 - 175


Details


Legend: Fig. 4. Separation of standard peptides, 1. CCK8 sulphated, 2. CCK8 desulphated, 3. gastrin-17 sulphated, 4. gastrin-17 desulphated.