107950 Synthesis of des-Pro2-bradykinin and its behavior in chromatography

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Synthesis of des-Pro2-bradykinin and its behavior in chromatography

Summary

Type: HPLC
Application #: 107950
Catalog#: 720120.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x150mm
Detection: UV, 210nm
Conditions: eluent: 10mM H3PO4 / K2HPO4 buffer pH 2.4 containing 50mM Na2SO4 - acetonitrile, gradient from 13 to 25% CH3CN in 23min
flow rate 1ml/min
Authors: Naruse, M. et al.
Source: Chem. Pharm. Bull. 29 (1981) 3734–3737


Details


Legend: Fig. 2. Separation of bradykinin derivatives by HPLC, A) des-Pro2-bradykinin, B) bradykinin, C) kallidin, D) Met-Lys-bradykinin, 2.2µg each in 2µl injected.