107870 Application of reversed phase HPLC in some critical peptide separations

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Application of reversed phase HPLC in some critical peptide separations

Summary

Type: HPLC
Application #: 107870
Catalog#: 720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection: UV, 210nm
Conditions: eluent A: 0.1% H3PO4, eluent B: H2O - CH3OH (50:50) with 0.05M tetramethylammonium hydroxide and H3PO4 to pH 2.8, linear gradient 20 to 100% B in 35min, then isocratic 100% B for 5min
flow rate 2ml/min
temperature 20°C
Authors: Janssen PSL
Source: J Chromatog Sci 22 (1984) 234 - 238


Details


Legend: Fig. 3 a. Separation of Org 2766 from its D-His analogue, 1. D-His derivative, 2. Org 2766. Fig. 5 HPLC separation of the basic hexapeptide ACTH-(11-16), a) conditions as in fig. 3 a, b) eluent A