107830 Separation and identification of hydrophilic peptides in dairy products using FMOC derivatization

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Separation and identification of hydrophilic peptides in dairy products using FMOC derivatization

Summary

Type: HPLC
Application #: 107830
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV at 260 nm
Conditions: eluent A: ACN - 100 mM ammonium acetate buffer, pH 3.8 (20:80), eluent B: ACN - 100 mM ammonium acetate buffer, pH 4.2 (80 : 20), gradient from 30% to 80% B in 50 min, then isocratic at 100% B for 10 min (gradient started 10 min after injection)
flow rate 1.0 ml/min
temperature 40°C
Authors: Roturier JM
Source: J Chromatogr A 696 (1995) 209 - 217


Details


Legend: Fig. 1: Separation of a derivatized standard mixture of amino acids containing 2.5 µmol of each amino acids. Fig. 2: Separation of synthetic peptide derivatives (T-Q, A-Q, A-Q-T, G-V-S, V-Q, I-N, V