107790 Determination of biotinidase activity by liquid chromatography with fluorimetric detection

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Determination of biotinidase activity by liquid chromatography with fluorimetric detection

Summary

Type: HPLC
Application #: 107790
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence, excitation 276nm, emission 340nm
Conditions: eluent A: aqueous 0.1% trifluoroacetic acid, B: methanol, gradient programme from 100% A to 100% B in 26min
flow rate programme between 0.8 and 1ml/min to reduce column inlet pressure
Authors: Hayakawa K
Source: J Chromatogr 383 (1986) 148 - 152


Details


Legend: Fig. 1. Chromatograms of A) enzyme solution from serum partially purified on DEAE cellulose, B) serum sample, 1. p-aminobenzoic acid (PAB), 2. N-biotinyl-p-aminobenzoic acid (biot-PAB). For deter