107780 HPLC determination of four kinds of biotin-containing carboxylase

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HPLC determination of four kinds of biotin-containing carboxylase

Summary

Type: HPLC
Application #: 107780
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV, 260nm
Conditions: eluent A: 0.1M Na phosphate buffer, pH 2.1, eluent B: methanol - solvent A (80:20, v/v), linear gradient from A to B in 15min
flow rate 1.5ml/min
Authors: Oizumi, J. et al.
Source: J. Chromatogr. 529 (1990) 55–63


Details


Legend: Fig. 4. Propionyl-CoA carboxylase assay, A) boiled homogenizates (control), B) homogenizates (sample), S = substrate, propionyl-CoA, P = product, methylmalonyl-CoA.